Effect of Triclosan Exposure on Developmental Competence in Parthenogenetic Porcine Embryo during Preimplantation

International Journal of Molecular Sciences

Seong-Keun Cho¹  Deog-Bon Koo²  Hyo-Jin Park²  Hyo-Gu Kang³  Bo-Woong Sim³  Bong-Seok Song³  Sun-Uk Kim³  SooHyun Park³  MinJu Kim³  Pil-Soo Jeong³  Young-Ho Park³  Sanghoon Lee³  KyungSeob Lim³  Jong-Hee Lee⁴  SeungHwan Lee⁴ 

[1] Department of Animal Science, Life and Industry Convergence Research Institute (RICRI), College of Natural Resources & Life Science, Pusan National University, 1268-50 Samrangjin-ro, Samrangjin-eup, Miryang, Gyeongnam 50463, Korea;Department of Biotechnology, College of Engineering, Daegu University, 201 Daegudae-ro, Jillyang, Gyeongsan, Gyeongbuk 38453, Korea;Futuristic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, Chungcheongbuk-do 28116, Korea;National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk-do 28116, Korea;

关键词: triclosan;    parthenogenetic embryo;    developmental competence;    oxidative stress;    mitochondria dysfunction;   

DOI  :  10.3390/ijms21165790

来源: DOAJ

【 摘 要 】

Triclosan (TCS) is included in various healthcare products because of its antimicrobial activity; therefore, many humans are exposed to TCS daily. While detrimental effects of TCS exposure have been reported in various species and cell types, the effects of TCS exposure on early embryonic development are largely unknown. The aim of this study was to determine if TCS exerts toxic effects during early embryonic development using porcine parthenogenetic embryos in vitro. Porcine parthenogenetic embryos were cultured in in vitro culture medium with 50 or 100 µM TCS for 6 days. Developmental parameters including cleavage and blastocyst formation rates, developmental kinetics, and the number of blastomeres were assessed. To determine the toxic effects of TCS, apoptosis, oxidative stress, and mitochondrial dysfunction were assessed. TCS exposure resulted in a significant decrease in 2-cell rate and blastocyst formation rate, as well as number of blastomeres, but not in the cleavage rate. TCS also increased the number of apoptotic blastomeres and the production of reactive oxygen species. Finally, TCS treatment resulted in a diffuse distribution of mitochondria and decreased the mitochondrial membrane potential. Our results showed that TCS exposure impaired porcine early embryonic development by inducing DNA damage, oxidative stress, and mitochondrial dysfunction.

【 授权许可】

Unknown