Human acellular amniotic membrane incorporating exosomes from adipose-derived mesenchymal stem cells promotes diabetic wound healing

Stem Cell Research & Therapy

Chunfang Xiao¹  Ruosi Chen²  Jin Wang²  Zhexiang Fan²  Shune Xiao²  Zhiqi Hu²  Yong Miao² 

[1] Department of Obstetrics and Gynecology, Nan Fang Hospital, Southern Medical University;Department of Plastic Surgery, Nan Fang Hospital, Southern Medical University;

关键词: Adipose-derived mesenchymal stem cells;    Exosomes;    Diabetic wound healing;    Acellular amniotic membrane;   

DOI  :  10.1186/s13287-021-02333-6

来源: DOAJ

【 摘 要 】

Abstract Background Diabetic wounds threaten the health and quality of life of patients and their treatment remains challenging. ADSC-derived exosomes have shown encouraging results in enhancing diabetic wound healing. However, how to use exosomes in wound treatment effectively is a problem that needs to be addressed at present. Methods A diabetic mouse skin wound model was established. ADSC-derived exosomes (ADSC-Exos) were isolated, and in vitro application of exosomes was evaluated using human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs). After preparation and characterization of a scaffold of human acellular amniotic membrane (hAAM) loaded with ADSC-Exos in vitro, they were transplanted into wounds in vivo and wound healing phenomena were observed by histological and immunohistochemical analyses to identify the wound healing mechanism of the exosome-hAAM composites. Results The hAAM scaffold dressing was very suitable for the delivery of exosomes. ADSC-Exos enhanced the proliferation and migration of HDFs and promoted proliferation and tube formation of HUVECs in vitro. In vivo results from a diabetic skin wound model showed that the hAAM-Exos dressing accelerated wound healing by regulating inflammation, stimulating vascularization, and promoting the production of extracellular matrix. Conclusion Exosome-incorporated hAAM scaffolds showed great potential in promoting diabetic skin wound healing, while also providing strong evidence for the future clinical applications of ADSC-derived exosomes.

【 授权许可】

Unknown