Genetic transformation of the dinoflagellate chloroplast

eLife

Katrin Geisler¹  Adrian C Barbrook²  Imen Lassadi²  Isabel C Nimmo²  R Ellen R Nisbet²  Christopher J Howe²  Alison G Smith³  Manuel Aranda³  Ross F Waller⁴  Saul Purton⁵  Jit Ern Chen⁵ 

[1] Jeffrey Sachs Center on Sustainable Development, Sunway University, Bandar Sunway, Malaysia;Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom;Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom;Institute of Structural and Molecular Biology, University College London, London, United Kingdom;Red Sea Research Center, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia;

关键词: dinoflagellate;    Amphidinium;    chloroplast;    transformation;    zooxanthella;    coral reef;   

DOI  :  10.7554/eLife.45292

来源: DOAJ

【 摘 要 】

Coral reefs are some of the most important and ecologically diverse marine environments. At the base of the reef ecosystem are dinoflagellate algae, which live symbiotically within coral cells. Efforts to understand the relationship between alga and coral have been greatly hampered by the lack of an appropriate dinoflagellate genetic transformation technology. By making use of the plasmid-like fragmented chloroplast genome, we have introduced novel genetic material into the dinoflagellate chloroplast genome. We have shown that the introduced genes are expressed and confer the expected phenotypes. Genetically modified cultures have been grown for 1 year with subculturing, maintaining the introduced genes and phenotypes. This indicates that cells continue to divide after transformation and that the transformation is stable. This is the first report of stable chloroplast transformation in dinoflagellate algae.

【 授权许可】

Unknown