Transcriptomic Changes in Hot Spring Frog Tadpoles (Buergeria otai) in Response to Heat Stress

Frontiers in Ecology and Evolution

Masatoshi Matsunami¹  Ko-Huan Lee²  Jhan-Wei Lin³  Yu Lee⁴  Si-Min Lin⁴  Shohei Komaki⁵  Yen-Po Lin⁷  Takeshi Igawa⁸ 

[1] Department of Advanced Genomic and Laboratory Medicine, Graduate School of Medicine, University of the Ryukyus, Nishihara, Japan;Department of Biological Sciences, Macquarie University, Sydney, NSW, Australia;Department of Biology, National Museum of Natural Science, National Taiwan Normal University, Taipei, Taiwan;Department of Life Science, National Taiwan Normal University, Taipei, Taiwan;Division of Biomedical Information Analysis, Iwate Tohoku Medical Megabank Organization, Iwate Medical University, Yahaba, Japan;Division of Bioresource Science, Amphibian Research Center, Hiroshima University, Higashihiroshima, Japan;Division of Zoology, Endemic Species Research Institute, National Taiwan Normal University, Taipei, Taiwan;Program of Basic Biology and Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashihiroshima, Japan;

关键词: amphibia;    anura;    differentially expressed genes;    protein-protein interaction network;    RNA-seq;    high thermal tolerance;   

DOI  :  10.3389/fevo.2021.706887

来源: DOAJ

【 摘 要 】

Buergeria frog tadpoles exhibit high thermal tolerance and are occasionally found in water pools that temporarily exceed 40°C. With the aim of understanding how they can cope with the severe heat stress, we performed RNA-seq of three heat-treated (38°C) and three control (25°C) tadpoles and compared their transcriptomic profiles. We identified 382 differentially expressed transcripts. A protein-protein interaction (PPI) network analysis of these transcripts further identified hub proteins involved in protein degradation, stress granule assembly, and global suppression of DNA transcription and mRNA translation. Along with the avoidance behavior against high water temperature, these endurance mechanisms potentially support tadpoles to survive in high temperatures for short periods of time. Similar mechanisms may exist in many other amphibian species whose habitats are prone to high temperatures.

【 授权许可】

Unknown