Characterization and Function of a Novel Welan Gum Lyase From Marine Sphingomonas sp. WG

Frontiers in Microbiology

Hui Li¹  Ai-Ping Chang²  Qiao-Mei He²  Ying-Lu Wang²  Hu Zhu²  Jie-Ying Lin²  Jin Qian³  Ya-Ling Shen³ 

[1] Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, China;Engineering Research Center of Industrial Biocatalysis, Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fujian Provincial Key Laboratory of Polymer Materials, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Chemistry and Materials Science, Fujian Normal University, Fuzhou, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China;

关键词: Sphingomonas sp. WG;    welan gum;    polysaccharide lyase;    characterization;    function;   

DOI  :  10.3389/fmicb.2021.638355

来源: DOAJ

【 摘 要 】

Welan gum, a kind of microbial exopolysaccharides, produced by the genus Sphingomonas, have great potential for application in many fields, such as the food industry, cement production, and enhanced oil recovery. But there are still challenges to reduce the cost, enhance the production and the quality. Herein, the bioinformatics analysis of WelR gene was preformed, and the characterization and function of WelR, welan gum lyase, from Sphingomonas sp. WG were investigated for the first time. The results indicated that 382nd (Asn), 383rd (Met), 494th (Asn), and 568th (Glu) were the key amino acid residues, and C-terminal amino acids were essential to keeping the stability of WelR. The optimal temperature and pH of the enzymatic activity were found to be 25°C and 7.4, respectively. And WelR was good low temperature resistance and alkali resistant. K+, Mg2+, Ca2+, Mn2+, and EDTA increased WelR activities, in contrast to Zn2+. Coupled with the change in glucose concentration and growth profile, the qRT-PCR results indicated that WelR may degrade welan gum existing in the culture to maintain bacterial metabolism when glucose was depleted. This work will lay a theoretical foundation to establish new strategies for the regulation of welan gum biosynthesis.

【 授权许可】

Unknown