Naphthalene degradation studies using Pseudomonas sp. strain SA3 from Alang-Sosiya ship breaking yard, Gujarat

Heliyon

Shristi Ram¹  Sandhya Mishra²  Sushma Rani Tirkey³ 

[1] Applied Phycology &Biotechnology Division, CSIR- Central Salt and Marine Chemicals Research Institute, Bhavnagar, 364002, India;Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India;

关键词: Naphthalene;    Biodegradation;    Polycyclic aromatic hydrocarbons (PAHs);    Alang-Sosiya ship breaking yard;    Pseudomonas sp.;   

DOI  :  

来源: DOAJ

【 摘 要 】

Polycyclic aromatic hydrocarbons (PAHs) remediation has received considerable attention due to their significant health concern and environmental pollution. However, PAHs contaminated sites also contain indigenous microbes that can potentially degrade naphthalene. Therefore, this study aimed to isolate, characterise and optimise process parameters for efficient naphthalene degradation. A total of 50 naphthalene degrading bacteria were isolated from Alang-Sosiya ship breaking yard, Bhavnagar, Gujarat and screened for their naphthalene degrading capacity. The selected isolate, Pseudomonas sp. strain SA3 was found to degrade 98.74 ± 0.00% naphthalene at a concentration of 500 ppm after 96 h. Further, optimisation of environmental parameters using one factor at a time approach using different inoculum sizes (v/v), pH, salinity, temperature, carbon and nitrogen source greatly accelerated the degradation process attaining 98.6 ± 0.46% naphthalene degradation after 72 h. The optimised parameters for maximum naphthalene degradation were pH 8, 0.1% peptone as nitrogen source, 8% salinity and 1% (v/v) inoculum size.

【 授权许可】

Unknown