Tongue microbiome in children with autism spectrum disorder

Journal of Oral Microbiology

Ahmed Abdulhaq¹  Vinod Kumar Basode¹  Husham E. Homeida²  Esam Halboub²  Tsute Chen³  Khalid Ammash Zain⁴  Ahmad Hassn Ghzwani⁴  Divyashri Baraniya⁵  Nezar Noor Al-Hebshi⁵ 

[1] College of Applied Medical Sciences, Jazan University;College of Dentistry, Jazan University;Forsyth Institute;Medical Research Centre, Jazan University;Oral Microbiome Research Laboratory, Maurice H. Kornberg School of Dentistry, Temple University;

关键词: autism spectrum disorder;    bacteria;    high-throughput nucleotide sequencing;    mouth;    microbiota;    tongue;   

DOI  :  10.1080/20002297.2021.1936434

来源: DOAJ

【 摘 要 】

Background: A few recent studies have characterized the salivary microbiome in association with Autism Spectrum Disorder (ASD). Here, we sought to assess if there is an association between the tongue microbiome and ASD. Methods: Tongue scrapping samples were obtained from 25 children with ASD and 38 neurotypical controls. The samples were sequenced for the 16S rRNA gene (V1-V3) and the resultant high-quality reads were assigned to the species-level using our previously described BLASTn-based algorithm. Downstream analyses of microbial profiles were conducted using QIIME, LEfSe, and R. Results: Independent of grouping, Prevotella, Streptococcus, Leptotrichia, Veillonella, Haemophilus and Rothia accounted for > 60% of the average microbiome. Haemophilus parainfluenzae, Rothia mucilaginosa, Prevotella melaninogenica and Neisseria flavescens/subflava were the most abundant species. Species richness and diversity did not significantly differ between the study groups. Thirteen species and three genera were differentially abundant between the two groups, e.g. enrichment of Actinomyces odontolyticus and Actinomyces lingnae and depletion of Campylobacter concisus and Streptococcus vestibularis in the ASD group. However, none of them withstood adjustment for multiple comparisons. Conclusion: The tongue microbiome of children with ASD was not significantly different from that of healthy control children, which is largely consistent with results from the literature.

【 授权许可】

Unknown