Antioxidant Potential of Sulfated Polysaccharides from Padina boryana; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model

Marine Drugs

Lei Wang¹  K.K. Asanka Sanjeewa¹  ThilinaU. Jayawardena¹  You-Jin Jeon¹  SangIn Kang²  Jung-Suck Lee³ 

[1] Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Korea;Department of Seafood and Aquaculture Science, Gyeongsang National University, Tongyeong 53064, Korea;Research Center for Industrial Development of Seafood, Gyeongsang National University, Tongyeong 53064, Korea;

关键词: antioxidant;    Maldives;    Padina boryana;    sulfated polysaccharide;    zebrafish;   

DOI  :  10.3390/md18040212

来源: DOAJ

【 摘 要 】

Elevated levels of reactive oxygen species (ROS) damage the internal cell components. Padina boryana, a brown alga from the Maldives, was subjected to polysaccharide extraction. The Celluclast enzyme assisted extract (PBE) and ethanol precipitation (PBP) of P. boryana were assessed against hydrogen peroxide (H₂O₂) induced cell damage and zebra fish models. PBP which contains the majority of sulfated polysaccharides based on fucoidan, showed outstanding extracellular ROS scavenging potential against H₂O₂. PBP significantly declined the intracellular ROS levels, and exhibited protection against apoptosis. The study revealed PBPs’ ability to activate the Nrf2/Keap1 signaling pathway, consequently initiating downstream elements such that catalase (CAT) and superoxide dismutase (SOD). Further, ROS levels, lipid peroxidation values in zebrafish studies were declined with the pre-treatment of PBP. Collectively, the results obtained in the study suggest the polysaccharides from P. boryana might be a potent source of water soluble natural antioxidants that could be sustainably utilized in the industrial applications.

【 授权许可】

Unknown