期刊论文详细信息
iScience | |
Single antibody detection in a DNA origami nanoantenna | |
Francesco Ricci1  Florian Steiner2  Simona Ranallo2  Viktorija Glembockyte3  Martina Pfeiffer3  Philip Tinnefeld3  Fiona Cole3  Kateryna Trofymchuk3  | |
[1]Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, CA 93106, USA | |
[2]Department of Chemical Science and Technologies, University of Rome, Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italy | |
[3]Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 München, Germany | |
关键词: Physical chemistry; Optics; Nanotechnology; Biochemistry; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Summary: DNA nanotechnology offers new biosensing approaches by templating different sensor and transducer components. Here, we combine DNA origami nanoantennas with label-free antibody detection by incorporating a nanoswitch in the plasmonic hotspot of the nanoantenna. The nanoswitch contains two antigens that are displaced by antibody binding, thereby eliciting a fluorescent signal. Single-antibody detection is demonstrated with a DNA origami integrated anti-digoxigenin antibody nanoswitch. In combination with the nanoantenna, the signal generated by the antibody is additionally amplified. This allows the detection of single antibodies on a portable smartphone microscope. Overall, fluorescence-enhanced antibody detection in DNA origami nanoantennas shows that fluorescence-enhanced biosensing can be expanded beyond the scope of the nucleic acids realm.【 授权许可】
Unknown