Single antibody detection in a DNA origami nanoantenna

iScience

Francesco Ricci¹  Florian Steiner²  Simona Ranallo²  Viktorija Glembockyte³  Martina Pfeiffer³  Philip Tinnefeld³  Fiona Cole³  Kateryna Trofymchuk³ 

[1] Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, CA 93106, USA;Department of Chemical Science and Technologies, University of Rome, Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italy;Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 München, Germany;

关键词: Physical chemistry;    Optics;    Nanotechnology;    Biochemistry;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: DNA nanotechnology offers new biosensing approaches by templating different sensor and transducer components. Here, we combine DNA origami nanoantennas with label-free antibody detection by incorporating a nanoswitch in the plasmonic hotspot of the nanoantenna. The nanoswitch contains two antigens that are displaced by antibody binding, thereby eliciting a fluorescent signal. Single-antibody detection is demonstrated with a DNA origami integrated anti-digoxigenin antibody nanoswitch. In combination with the nanoantenna, the signal generated by the antibody is additionally amplified. This allows the detection of single antibodies on a portable smartphone microscope. Overall, fluorescence-enhanced antibody detection in DNA origami nanoantennas shows that fluorescence-enhanced biosensing can be expanded beyond the scope of the nucleic acids realm.

【 授权许可】

Unknown