Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation

Antioxidants

MichaelR. Volkert¹  Hemant Khanna²  Keith Reddig³  Gregory Hendricks³  Saumya Jakati⁴  RishikeshKumar Gupta⁵  Sushma Vishwakarma⁵  Inderjeet Kaur⁵  Mudit Tyagi⁶  Jay Chhablani⁶  RajeevReddy Pappuru⁶ 

[1] Department of Microbiology & Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01655, USA;Department of Ophthalmology & Visual Sciences, University of Massachusetts Medical School, Worcester, MA 01655, USA;Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA;Ophthalmic Pathology Laboratory, L.V. Prasad Eye Institute, Hyderabad 500034, India;Prof Brien Holden Eye Research Centre, LV Prasad Eye Institute, Hyderabad 500034, India;Smt. Kanuri Santhamma Retina Vitreous Centre, L.V. Prasad Eye Institute, Hyderabad 500034, India;

关键词: human retina;    epiretinal membrane;    internal limiting membrane;    vitreoretinal surgery;    macular hole;    proliferative diabetic retinopathy;   

DOI  :  10.3390/antiox9080654

来源: DOAJ

【 摘 要 】

Fibrocellular membrane or epiretinal membrane (ERM) forms on the surface of the inner limiting membrane (ILM) in the inner retina and alters the structure and function of the retina. ERM formation is frequently observed in ocular inflammatory conditions, such as proliferative diabetic retinopathy (PDR) and retinal detachment (RD). Although peeling of the ERM is used as a surgical intervention, it can inadvertently distort the retina. Our goal is to design alternative strategies to tackle ERMs. As a first step, we sought to determine the composition of the ERMs by identifying the constituent cell-types and gene expression signature in patient samples. Using ultrastructural microscopy and immunofluorescence analyses, we found activated microglia, astrocytes, and Müller glia in the ERMs from PDR and RD patients. Moreover, oxidative stress and inflammation associated gene expression was significantly higher in the RD and PDR membranes as compared to the macular hole samples, which are not associated with inflammation. We specifically detected differential expression of hypoxia inducible factor 1-α (HIF1-α), proinflammatory cytokines, and Notch, Wnt, and ERK signaling pathway-associated genes in the RD and PDR samples. Taken together, our results provide new information to potentially develop methods to tackle ERM formation.

【 授权许可】

Unknown