Development and validation of fluoroquinolone detection method in chicken eggs using high-performance liquid chromatography

Ветеринария сегодня

T. B. Nikeshina¹  A. A. Lugovskoy¹  D. S. Bolshakov¹ 

[1] ФГБУ «ВНИИЗЖ», г. Владимир;

关键词: фторхинолоны;    антибиотики;    куриные яйца;    высокоэффективная жидкостная хроматография;    флуориметрическое детектирование;    валидация;    fluoroquinolones;    antibiotics;    chicken eggs;    high-performance liquid chromatography;    fluorescence detection;    validation;   

DOI  :  

来源: DOAJ

【 摘 要 】

The method for the simultaneous detection of nine fluoroquinolone antibiotics (marbofloxacin, ofloxacin, pefloxacin, norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin, sarafloxacin and difloxacin) in chicken eggs using high-performance liquid chromatography with fluorescence detection was developed. The detected range of fluoroquinolones is from 10 to 100 μg/kg in a 4g sample. Based on the validation studies the combined standard uncertainty and relative expanded uncertainty were calculated. The technique enables to detect antibiotic residues in their admissible levels equal to 100 μg/kg, in compliance with SanPiN 2.3.2.2804-10 and Common sanitary-epidemiological and hygienic requirements to the goods subject to sanitary and epidemiological surveillance (control).

【 授权许可】

Unknown