Melatonin Hormone Acts on Cells of Maternal Blood and Placenta From Diabetic Mothers

Frontiers in Physiology

Iracema de Mattos Paranhos Calderon¹  Eduardo Luzía França²  Adriele Athaídes Queiroz²  Adenilda Cristina Honorio-França²  Martino B. Pierre Louis²  Danielle Cristina Honorio França³ 

[1] Gynecology, Obstetrics and Mastology Posgraduate Programme, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, Brazil;Institute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças, Brazil;Medical Course, State University of Mato Grosso, Cáceres, Brazil;

关键词: apoptosis;    mononuclear cells;    diabetes;    melatonin;    placenta;    blood;   

DOI  :  10.3389/fphys.2021.765928

来源: DOAJ

【 摘 要 】

Changes in glucose metabolism of diabetic mothers affect immunological components, proinflammatory factors, and placental hypervascularization that can induce cell death. The hormone melatonin has been identified as a potential modulating agent. The aim of this study was to analyze the oxidative process and the apoptosis in maternal blood and placental cells modulated by melatonin from diabetic mothers. The groups were 40 pregnant women divided into non-diabetic (ND) and type 2 diabetes mellitus (T2DM) groups. Blood and placental cells were obtained by density gradient and maintained in culture treated or not with melatonin (100 ng/mL) for 24 h (37°C, 5% CO2). Oxidative stress was evaluated by superoxide release and CuZn superoxide dismutase (SOD). Apoptosis was assessed by flow cytometry. Maternal hyperglycemia increased superoxide release and apoptosis in MN cells from maternal blood and reduced SOD level and SOD/O2- ratio. Melatonin reduced oxidative stress and apoptosis rates in MN cells in the blood of diabetic mothers. There was a reduction in SOD and SOD/O2- ratio in the placental extravillous layer, and melatonin restored the concentrations of this enzyme. There was greater superoxide release, reduced SOD/O2- ratio, and apoptosis in MN cells placental villous layer. Melatonin increased apoptosis rates in the placental villous layer from hyperglycemic mothers. These data suggest that hyperglycemia altered the processes oxidative in blood and placenta from hyperglycemic mothers. These changes reflected in the mechanisms of induction of apoptosis, especially in the vascularized layers of the placenta, and were modulated by melatonin.

【 授权许可】

Unknown