Effects of 4-nonylphenol on oxidant/antioxidant balance system inducing hepatic steatosis in male rat

Toxicology Reports

Ansoumane Kourouma¹  Peng Duan¹  Suqin Qi¹  Kedi Yang¹  Chao Quan¹  Koikoi Kebe Bilivogui²  Ndjiembi Adjonga Christiane³  Aidogie Osamuyimen³  Hady Keita⁴ 

[1] MOE Key Laboratory of Environmental and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, People’s Republic of China;School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, People’s Republic of China;School of Medicine and Health Management, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, People’s Republic of China;Universidad Tecnológica del Valle de Toluca, Carretera del Departamento del D.F., km 7.5. Santa María Atarasquillo Lerma, Mexico;

关键词: 4-Nonylphenol;    Hepatic steatosis;    Oxidative stress;    Genes;    Liver;    Apoptosis;   

DOI  :  10.1016/j.toxrep.2015.10.006

来源: DOAJ

【 摘 要 】

An emerging literature suggests that early life exposure to 4-nonylphenol (4-NP), a widespread endocrine disrupting chemical, may increase the risk of metabolic syndrome. In this study, we investigated the hypothesis that intraperitoneal administration of 4-NP induces hepatic steatosis in rat. 24 male Sprague-Dawley rats were administered with 4-NP (0, 2, 10 and 50 mg/kg b.wt) in corn oil for 30 days. Liver histology, biochemical analysis and gene expression profiling were examined. After treatment, abnormal liver morphology and function were observed in the 4-NP-treated rat, and significant changes in gene expression an indicator of hepatic steatosis and apoptosis were observed compared with controls. Up-regulated genes involved in apoptosis, hepatotoxity and oxidative stress, increased ROS and decrease of antioxidant enzyme were observed in the 4-NP exposed rat. Extensive fatty accumulation in liver section and elevated serum GOT, GPT, LDH and γ-GT were also observed. Incidence and severity of liver steatosis was scored and taken into consideration (steatosis, ballooning and lobular inflammation). Hepatocytes apoptosis could promote NAFLD progression; Fas/FasL, TNF-α and Caspase-9 mRNA activation were important contributing factors to hepatic steatosis. These findings provide the first evidence that 4-NP affects the gene expression related to liver hepatotoxicity, which is correlated with hepatic steatosis.

【 授权许可】

Unknown