Heat Stress Pre-Exposure May Differentially Modulate Plant Defense to Powdery Mildew in a Resistant and Susceptible Barley Genotype

Genes

Viola Kunos¹  Klára Mészáros¹  Judit Bányai¹  Lóránt Király²  Judit Kolozsváriné Nagy²  József Fodor²  András Künstler²  Ildikó Schwarczinger² 

[1] Centre for Agricultural Research, Agricultural Institute, ELKH, 2 Brunszvik Str., H-2462 Martonvásár, Hungary;Centre for Agricultural Research, Plant Protection Institute, ELKH, 15 Herman Ottó Str., H-1022 Budapest, Hungary;

关键词: barley;    BAX inhibitor;    combined stress resistance;    heat stress;    pathogenesis-related genes;    plant defense;   

DOI  :  10.3390/genes12050776

来源: DOAJ

【 摘 要 】

Heat stress negatively affects barley production and under elevated temperatures defense responses to powdery mildew (Blumeria graminis f. sp. hordei, Bgh) are altered. Previous research has analyzed the effects of short-term (30 s to 2 h) heat stress, however, few data are available on the influence of long-term exposure to heat on powdery mildew infections. We simultaneously assessed the effects of short and long term heat pre-exposure on resistance/susceptibility of barley to Bgh, evaluating powdery mildew infection by analyzing symptoms and Bgh biomass with RT-qPCR in barley plants pre-exposed to high temperatures (28 and 35 °C from 30 s to 5 days). Plant defense gene expression after heat stress pre-exposure and inoculation was also monitored. Our results show that prolonged heat stress (24, 48 and 120 h) further enhanced Bgh susceptibility in a susceptible barley line (MvHV118-17), while a resistant line (MvHV07-17) retained its pathogen resistance. Furthermore, prolonged heat stress significantly repressed the expression of several defense-related genes (BAX inhibitor-1, Pathogenesis related-1b and Respiratory burst oxidase homologue F2) in both resistant and susceptible barley lines. Remarkably, heat-suppressed defense gene expression returned to normal levels only in MvHV07-17, a possible reason why this barley line retains Bgh resistance even at high temperatures.

【 授权许可】

Unknown