Activities of α-galactosidase and polygalacturonase during hydration of Dalbergia nigra ((Vell.) Fr All. ex Benth.) seeds at different temperatures

Journal of Seed Science

Andressa Vasconcelos Flores¹  José Francisco de Carvalho Gonçalves²  Elisa Monteze Bicalho³  Eduardo Euclydes de Lima e Borges⁴  Glauciana da Mata Ataíde⁴  Valéria Monteze Guimarães⁴ 

[1] Instituto Federal de Educação, Ciência e Tecnologia de Mato Grosso;Instituto Nacional de Pesquisas da Amazônia;Universidade Federal de Minas Gerais;Universidade Federal de Viçosa;

关键词: embebição;    jacarandá-da-bahia;    modificações bioquímicas;    monossacarídeos;   

DOI  :  

来源: DOAJ

【 摘 要 】

Germination is a process that begins with seed water uptake, stimulating enzyme synthesis or activating enzymes already present. The objective of this study was to evaluate variations in monosaccharide reserves and the activities of the α-galactosidase and polygalacturonase enzymes during the hydration of two lots of Dalbergia nigra (Bahia Rosewood) seeds. Seeds from different origins constituted the two lots I and II, classified as high and low vigor, respectively. Both lots were placed in desiccators with a high relative humidity to hydrate and at 15 and 25 °C until levels of 10, 15, 20 and 25% moisture levels in seeds were reached. The seed cotyledons were analyzed for the quantity of monosaccharides and enzyme activity. The control had higher concentrations of xylose and rhamnose, which decreased during hydration until the 15% level was reached, after which concentrations increased again in both lots. Lot I, with a superior quality, showed higher glucose synthesis and degradation during seed hydration. Both enzymes were pre-existing since activity was already present in the seeds without imbibition. The polygalacturonase enzyme increased and the α-galactosidase enzyme remained relatively constant during seed hydration.

【 授权许可】

Unknown