Beyond Single-Cell Analysis of Metallodrugs by ICP-MS: Targeting Cellular Substructures

International Journal of Molecular Sciences

Nicola Lüdi¹  Peter Broekmann¹  Martin Nils Hungerbühler²  Christoph Kempf²  Hendrik von Tengg-Kobligk²  Audrey Galé²  Johannes Thomas Heverhagen²  Nico Ruprecht²  Lukas Hofmann³ 

[1] Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP), University of Bern, 3012 Bern, Switzerland;Department of Diagnostic, Interventional and Pediatric Radiology, Bern University Hospital, University of Bern, 3010 Bern, Switzerland;Institute of Nanotechnology and Advanced Materials & Department of Chemistry, Faculty of Exact Sciences, Bar-Ilan University, Ramat-Gan 5290002, Israel;

关键词: NSCLC;    cisplatin;    chemotherapy;    single cell ICP-MS;    cisplatin resistance;    nuclear uptake;   

DOI  :  10.3390/ijms22179468

来源: DOAJ

【 摘 要 】

Platinum compounds such as cisplatin (cisPt) embody the backbone of combination chemotherapy protocols against advanced lung cancer. However, their efficacy is primarily limited by inherent or acquired platinum resistance, the origin of which has not been fully elucidated yet, although of paramount interest. Using single cell inductively coupled plasma mass spectrometry (SC-ICP-MS), this study quantifies cisPt in single cancer cells and for the first time in isolated nuclei. A comparison of cisPt uptake was performed between a wild type (wt) cancer cell line and related resistant sublines. In both, resistant cells, wt cells, and their nuclei, cisPt uptake was measured at different incubation times. A lower amount of cisPt was found in resistant cell lines and their nuclei compared to wt cells. Moreover, the abundance of internalized cisPt decreased with increasing resistance. Interestingly, concentrations of cisPt found within the nuclei were higher than compared to cellular concentrations. Here, we show, that SC-ICP-MS allows precise and accurate quantification of metallodrugs in both single cells and cell organelles such as nuclei. These findings pave the way for future applications investigating the potency and efficacy of novel metallodrugs developed for cancer treatment.

【 授权许可】

Unknown