Ribosomal DNA internal transcribed spacer 2 sequence analysis and phylogenetic comparison of seven cockroach species in northwestern Iran

BMC Research Notes

Mostafa Farmani¹  Saber Gholizadeh¹  Hamidreza Basseri²  Behzad Norouzi³ 

[1] Cellular and Molecular Research Center, Urmia University of Medical Sciences;Medical Entomology Department, School of Public Health, Tehran University of Medical Sciences;Research Center of Health and Environment, Guilan University of Medical Sciences;

关键词: rDNA-ITS2;    Phylogeny;    Blattidae;    Ectobiidae;    Corydiidae;   

DOI  :  10.1186/s13104-019-4089-3

来源: DOAJ

【 摘 要 】

Abstract Objectives The current study was conducted to identify cockroach species (Blattodea) of northwestern Iran in public places using morphological characteristics and ribosomal DNA internal transcribed spacer 2 (rDNA-ITS2). Sequences were analyzed with Basic Local Alignment Search Tool (BLAST) searches, Neighbor-Joining methods based on and Tamura-Nei phylogenetic analyses. In addition, eight cockroach rDNA-ITS2 sequences from China, India, Iran and the United States obtained from GenBank were compared to those obtained in this study. Results Specimens collected in Iran were identified as Periplaneta americana (L.), Shelfordella lateralis (Walker), Blatta orientalis (L.) (Blattodea: Blattidae), Blattella germanica (L.), Supella longipalpa (F.) (Blattodea: Ectobiidae), Polyphaga aegyptiaca (L.), and Polyphaga saussurei (Dohrn) (Blattodea: Corydiidae). rDNA-ITS2 nucleotide sequence analysis showed 100% similarity between P. aegyptiaca and P. saussurei species collected from Iran despite morphological differences. However, ITS2 sequence of P. americana submitted from China showed 30.49–31.71% difference to P. americana sequences from Iran and the United States. The results highlight the importance of morphological identification of cockroach species before conducting molecular techniques.

【 授权许可】

Unknown