Advanced Optogenetic-Based Biosensing and Related Biomaterials

Materials

Mihaela Gheorghiu¹  Cristina Polonschii¹  Eugen Gheorghiu¹  Octavian Popescu² 

[1] International Centre of Biodynamics, 1B Intrarea Portocalelor, 060101 Bucharest, Romania;Molecular Biology Center, Institute for Interdisciplinary Research in Bio-Nano-Sciences, Babes-Bolyai-University, 400084 Cluj-Napoca, Romania;

关键词: optogenetic (light-responsive) biomaterials;    cell-based biosensors;    time-lapse multiparametric assays;    optogenetic control;    cell dynamics;   

DOI  :  10.3390/ma14154151

来源: DOAJ

【 摘 要 】

The ability to stimulate mammalian cells with light, brought along by optogenetic control, has significantly broadened our understanding of electrically excitable tissues. Backed by advanced (bio)materials, it has recently paved the way towards novel biosensing concepts supporting bio-analytics applications transversal to the main biomedical stream. The advancements concerning enabling biomaterials and related novel biosensing concepts involving optogenetics are reviewed with particular focus on the use of engineered cells for cell-based sensing platforms and the available toolbox (from mere actuators and reporters to novel multifunctional opto-chemogenetic tools) for optogenetic-enabled real-time cellular diagnostics and biosensor development. The key advantages of these modified cell-based biosensors concern both significantly faster (minutes instead of hours) and higher sensitivity detection of low concentrations of bioactive/toxic analytes (below the threshold concentrations in classical cellular sensors) as well as improved standardization as warranted by unified analytic platforms. These novel multimodal functional electro-optical label-free assays are reviewed among the key elements for optogenetic-based biosensing standardization. This focused review is a potential guide for materials researchers interested in biosensing based on light-responsive biomaterials and related analytic tools.

【 授权许可】

Unknown