Jaw osteosarcoma models in mice: first description

Journal of Translational Medicine

Romain Guilho¹  Dominique Heymann²  Françoise Rédini³  Hélios Bertin³  Séverine Battaglia³  Régis Brion³  Jérôme Amiaud³  Julie Longis⁴  Benoit Piot⁴  Pierre Corre⁴  Anne Moreau⁵  Anne Brouchet-Gomez⁶ 

[1] Faculty of Population Health Sciences, UCL Institute of Child Health;Laboratoire Hétérogénéité Tumorale et Médecine de Précision, Institut de Cancérologie de l’Ouest;Laboratoire des sarcomes osseux et remodelage des tissus calcifiés (Phy.OS), UMR 1238, Faculté de médecine;Service de chirurgie Maxillo-faciale et stomatologie, CHU de Nantes;Service d’anatomie et cytologie pathologique, CHU de Nantes;Service d’anatomie et cytologie pathologique, Institut Universitaire du Cancer Toulouse Oncopôle;

关键词: Sarcoma;    Mandible;    Models;    Environment;    Animal experimentation;    Bone resorption;   

DOI  :  10.1186/s12967-019-1807-5

来源: DOAJ

【 摘 要 】

Abstract Background Osteosarcoma (OS) is the most common cancer of bone. Jaw osteosarcoma (JOS) is rare and it differs from other OS in terms of the time of occurrence (two decades later) and better survival. The aim of our work was to develop and characterize specific mouse models of JOS. Methods Syngenic and xenogenic models of JOS were developed in mice using mouse (MOS-J) and human (HOS1544) osteosarcoma cell lines, respectively. An orthotopic patient-derived xenograft model (PDX) was also developed from a mandibular biopsy. These models were characterized at the histological and micro-CT imaging levels, as well as in terms of tumor growth and metastatic spread. Results Homogeneous tumor growth was observed in both the HOS1544 and the MOS-J JOS models by injection of 0.25 × 106 and 0.50 × 106 tumor cells, respectively, at perimandibular sites. Histological characterization of the tumors revealed features consistent with high grade conventional osteosarcoma, and the micro-CT analysis revealed both osteogenic and osteolytic lesions. Early metastasis was encountered at day 14 in the xenogenic model, while there were no metastatic lesions in the syngenic model and in the PDX models. Conclusion We describe the first animal model of JOS and its potential use for therapeutic applications. This model needs to be compared with the usual long-bone osteosarcoma models to investigate potential differences in the bone microenvironment.

【 授权许可】

Unknown