Extraction and partial purification of coagulation active components from common bean seed

Acta Periodica Technologica

关键词: natural coagulant;    common bean seed;    purification;    water clarification;    coagulation activity;   

DOI  :  10.2298/APT0637037S

来源: DOAJ

【 摘 要 】

An active coagulation component was extracted from common bean seed by NaCl solution and the obtained crude extract was partially purified through a sequence of steps that included precipitation of protein by ammonium sulphate, desalting by dialysis and anion exchange. A turbid water was treated by protein fractions obtained in the anion- exchange elution process by stepwise increase in NaCl concentration. The jar tests were conducted at various dosages of eluates. Different mode of relation between coagulation activity and applied coagulant dose for each protein fraction indicated the existence of different mechanisms of coagulation/flocculation, depending of characteristics of different proteins in the fractions.

【 授权许可】

Unknown