The Ramazzini Institute 13-week pilot study on glyphosate and Roundup administered at human-equivalent dose to Sprague Dawley rats: effects on the microbiome

Environmental Health

Fabiana Manservisi¹  Daniele Mandrioli¹  Ilaria Menghetti¹  Fiorella Belpoggi¹  Luciano Bua¹  Simona Panzacchi¹  Laura Falcioni¹  Andrea Vornoli¹  Jia Chen²  Corina Lesseur²  Qixing Mao³  Jianzhong Hu³ 

[1] Cesare Maltoni Cancer Research Center (CMCRC), Ramazzini Institute (RI);Department of Environmental Medicine and Public Health, Icahn School of Medicine at Mount Sinai;Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai;

关键词: Roundup;    Glyphosate;    Gut microbiome;    Early developmental stage;   

DOI  :  10.1186/s12940-018-0394-x

来源: DOAJ

【 摘 要 】

Abstract Background Glyphosate-based herbicides (GBHs) are broad-spectrum herbicides that act on the shikimate pathway in bacteria, fungi, and plants. The possible effects of GBHs on human health are the subject of an intense public debate for both its potential carcinogenic and non-carcinogenic effects, including its effects on microbiome. The present pilot study examines whether exposure to GBHs at doses of glyphosate considered to be “safe” (the US Acceptable Daily Intake - ADI - of 1.75 mg/kg bw/day), starting from in utero, may modify the composition of gut microbiome in Sprague Dawley (SD) rats. Methods Glyphosate alone and Roundup, a commercial brand of GBHs, were administered in drinking water at doses comparable to the US glyphosate ADI (1.75 mg/kg bw/day) to F0 dams starting from the gestational day (GD) 6 up to postnatal day (PND) 125. Animal feces were collected at multiple time points from both F0 dams and F1 pups. The gut microbiota of 433 fecal samples were profiled at V3-V4 region of 16S ribosomal RNA gene and further taxonomically assigned and assessed for diversity analysis. We tested the effect of exposure on overall microbiome diversity using PERMANOVA and on individual taxa by LEfSe analysis. Results Microbiome profiling revealed that low-dose exposure to Roundup and glyphosate resulted in significant and distinctive changes in overall bacterial composition in F1 pups only. Specifically, at PND31, corresponding to pre-pubertal age in humans, relative abundance for Bacteriodetes (Prevotella) was increased while the Firmicutes (Lactobacillus) was reduced in both Roundup and glyphosate exposed F1 pups compared to controls. Conclusions This study provides initial evidence that exposures to commonly used GBHs, at doses considered safe, are capable of modifying the gut microbiota in early development, particularly before the onset of puberty. These findings warrant future studies on potential health effects of GBHs in early development such as childhood.

【 授权许可】

Unknown