Multi-Extraction and Quality of Protein and Carrageenan from Commercial Spinosum (Eucheuma denticulatum)

Foods

Tommy Ewi Pedersen¹  Karin Meyer Hansen¹  Jan Larsen¹  Jimmy J.P. Sejberg¹  Charlotte Jacobsen²  Alireza Naseri²  Susan L. Holdt² 

[1] Innovation, CP Kelco, Ved Banen 16, DK-4623 Lille Skensved, Denmark;Research Group for Bioactives—Analysis and Application, The National Food Institute, Technical University of Denmark (DTU Food), Kemitorvet, Building 204, DK-2800 Kgs. Lyngby, Denmark;

关键词: algae;    industrial seaweeds;    vegan protein;    combined extraction;    bioactive compounds;    sustainability;   

DOI  :  10.3390/foods9081072

来源: DOAJ

【 摘 要 】

Seaweeds contain many valuable compounds that can be used in the food industry. Carrageenan is a polysaccharide which has been extracted from seaweed for centuries and is used as a texturizer in food and non-food products. However, seaweeds contain compounds other than carrageenan, such as proteins, which could also be extracted. This extraction should be done without compromising the industrial scale carrageenan extraction yield and quality. This study aimed at up-stream protein extraction from red seaweed Eucheuma denticulatum by using of an optimized enzyme-assisted extraction, including of an aqueous/enzymatic treatment followed by alkaline extraction, and then the commercial carrageenan extraction. The protein extraction efficiency of four enzymes was evaluated including Celluclast^® 1.5L, Shearzyme^® 500 L, Alcalase^® 2.4 L FG and Viscozyme^® L at a concentration of 0.0, 0.1, 0.2 and 0.4% (w/w). To avoid detrimental effects on carrageenan, all the experiments were performed at pH 7 at room temperature. The results showed that 0.2% w/w Alcalase^® or Viscozyme^® added individually achieved the highest protein extraction efficiencies (59 and 48%, respectively) at pH 7 and room temperature (p < 0.05). Determination of the most common carrageenan quality parameters indicated that using any of these enzymes had no negative effect on the carrageenan yield and quality.

【 授权许可】

Unknown