Rhynchosia volubilis Promotes Cell Survival via cAMP-PKA/ERK-CREB Pathway

Pharmaceuticals

Gyu-Ho Choi¹  Eunhye Kim¹  Yoon-Kwan Jang¹  Tae-Jin Kim¹  Heon-Su Kim¹  Sang-Hyun Ahn¹  Jung-Soo Suh¹ 

[1] Department of Integrated Biological Science, Pusan National University, Pusan 46241, Korea;

关键词: Rhynchosia volubilis;    FRET;    cAMP;    PKA;    CREB;   

DOI  :  10.3390/ph15010073

来源: DOAJ

【 摘 要 】

Rhynchosia volubilis, a small black bean, has been used as a traditional remedy to treat diseases and maintain health in East Asia, but its cellular effects and molecular mechanisms are not fully understood. The purpose of this study was to investigate the effect of ethanol extract from Rhynchosia volubilis (EERV) on cell survival and to elucidate the biochemical signaling pathways. Our results showed that EERV stimulated the cyclic AMP (cAMP) signal revealed by a fluorescent protein (FP)-based intensiometric sensor. Using a Förster resonance energy transfer (FRET)-based sensor, we further revealed that EERV could activate PKA and ERK signals, which are downstream effectors of cAMP. In addition, we reported that EERV could induce the phosphorylation of CREB, a key signal for cell survival. Thus, our results suggested that EERV protects against apoptosis by activating the cell survival pathway through the cAMP-PKA/ERK-CREB pathway.

【 授权许可】

Unknown