Effect of temperature on pollen germination for several Rosaceae species: influence of freezing conservation time on germination patterns

PeerJ

Francisco García Breijo¹  Hugo Merle¹  Carlos Zornoza¹  Nuria Cebrián¹  Aina Valls¹  Roberto Beltrán¹  Alfonso Garmendia²  José Reig Armiñana³ 

[1] Departamento de Ecosistemas Agroforestales, Universitat Politècnica de València, Valencia, Spain;Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Valencia, Valencia, Spain;Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universidad de Valencia, Valencia, Spain;

关键词: Pollen germination;    Pollen tube length;    Almond;    Apple;    Cherry;    Peach;   

DOI  :  10.7717/peerj.8195

来源: DOAJ

【 摘 要 】

Between February 2018 and April 2018, flowers were collected from eight Rosaceae species. Flowers were kept in a freezer at −20 °C for three freezing times (Treatment 1, two months; Treatment 2, four months; Treatment 3, six months). After extracting pollen, in vitro germination was induced in a culture medium and incubated at six different temperatures for 72 h. The percentage of pollen germination, average pollen tube length and maximum pollen tube length were measured. Pollen germination was maximum for all species between 15 °C and 30 °C. Cydonia oblonga, Malus sylvestris, Prunus avium, Prunus domestica, Prunus dulcis, Prunus persica and Pyrus communis obtained 30–52% pollen germination between 15 °C and 20 °C. Prunus cerasifera had 40% pollen germination at 30 °C. All species studied reached the maximum pollen tube length between 10 °C and 25 °C. Germination did not change significantly for any of the species with freezing time, but we found significant differences in the three parameters measured between treatments. The highest germination percentages were obtained in Treatment 2 (four months frozen at −20 °C), while the maximum pollen tube length was reached in Treatment 1 (two months frozen at −20 °C). According to our results, freezing time affected the germination-temperature patterns. This could indicate that studies on the effect of temperature on pollen germination should always be carried out with fresh pollen to obtain more conclusive data.

【 授权许可】

Unknown