Genetic Diversity of Hatchery-Bred Brown Trout (Salmo trutta) Compared with the Wild Population: Potential Effects of Stocking on the Indigenous Gene Pool of a Norwegian Reservoir

Diversity

Arne N. Linløkken¹  Wenche Johansen²  Stein I. Johnsen³ 

[1] Faculty of Applied Ecology, Agricultural Sciences and Biotechnology, Campus Evenstad, Inland Norway University of Applied Sciences, N-2418 Elverum, Norway;Faculty of Applied Ecology, Agricultural Sciences and Biotechnology, Campus Hamar, Inland Norway University of Applied Sciences, N-2418 Elverum, Norway;Norwegian Institute of Nature Research, Department Lillehammer, Vormstuguvegen 40, N-2624 Lillehammer, Norway;

关键词: artificial breeding;    effective population size;    genetic diversity;    siblings;    bottleneck events;    genetic structure;   

DOI  :  10.3390/d13090414

来源: DOAJ

【 摘 要 】

This study was conducted in Lake Savalen in southeastern Norway, focusing on genetic diversity and the structure of hatchery-reared brown trout (Salmo trutta) as compared with wild fish in the lake and in two tributaries. The genetic analysis, based on eight simple sequence repeat (SSR) markers, showed that hatchery bred single cohorts and an age structured sample of stocked and recaptured fish were genetically distinctly different from each other and from the wild fish groups. The sample of recaptured fish showed the lowest estimated effective population size N_e = 8.4, and the highest proportion of siblings, despite its origin from five different cohorts of hatchery fish, counting in total 84 parent fish. Single hatchery cohorts, originating from 13–24 parental fish, showed N_e = 10.5–19.9, suggesting that the recaptured fish descended from a narrow group of parents. BayeScan analysis indicated balancing selection at several loci. Genetic indices of wild brown trout collected in the lake in 1991 and 2010 suggested temporal genetic stability, i.e., the genetic differentiation (F_ST) was non-significant, although the N_e, the number of alleles per locus and the number of private alleles were lower in the 2010 sample.

【 授权许可】

Unknown