CRISPR/Cas Technologies and Their Applications in Escherichia coli

Frontiers in Bioengineering and Biotechnology

Huina Dong¹  Yali Cui¹  Dawei Zhang³ 

[1] Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, China;Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China;University of Chinese Academy of Sciences, Beijing, China;

关键词: Escherichia coli;    CRISPR system;    transcriptional regulation;    knock-down;    knock-in;   

DOI  :  10.3389/fbioe.2021.762676

来源: DOAJ

【 摘 要 】

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have revolutionized genome editing and greatly promoted the development of biotechnology. However, these systems unfortunately have not been developed and applied in bacteria as extensively as in eukaryotic organism. Here, the research progress on the most widely used CRISPR/Cas tools and their applications in Escherichia coli is summarized. Genome editing based on homologous recombination, non-homologous DNA end-joining, transposons, and base editors are discussed. Finally, the state of the art of transcriptional regulation using CRISPRi is briefly reviewed. This review provides a useful reference for the application of CRISPR/Cas systems in other bacterial species.

【 授权许可】

Unknown