Single-Nucleotide Polymorphism Variations Associated With Specific Genes Putatively Identified Enhanced Genetic Predisposition for 305-Day Milk Yield in the Girolando Crossbreed

Frontiers in Genetics

Danilo Conrado Silva¹  Yuri Tani Utsunomiya²  José Fernando Garcia²  Marcos Vinicius Gualberto Barbosa da Silva³  Adriana Santana do Carmo⁴  Claudio Carlos da Silva⁵  Lysa Bernardes Minasi⁵  Aparecido Divino da Cruz⁵  Larissa Kamídia de Farias Teixeira⁵  Flávia Melo Rodrigues⁵  Alex Silva da Cruz⁵ 

[1] Curso de Graduação em Medicina Veterinária, Instituto Acadêmico de Ciências Agrárias e Sustentabilidade, Universidade Estadual de Goiás, São Luís de Montes Belos, Brazil;Departamento de Apoio a Produção e Saúde Animal, Faculdade de Medicina Veterinária, Universidade Estadual Paulista Júlio de Mesquita Filho, Araçatuba, Brazil;Empresa Brazileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa de Gado de Leite, Juiz de Fora, Brazil;Escola de Veterinária e Zootecnia, Departamento de Zootecnia, Universidade Federal de Goiás, Goiânia, Brazil;Mestrado em Genética, Núcleo de Pesquisas Replicon, Escola de Ciências Agrárias e Biológicas, Pontifícia Universidade Católica de Goiás, Goiânia, Brazil;

关键词: SNP;    dairy cattle;    indicine;    GWAS;    selection;   

DOI  :  10.3389/fgene.2020.573344

来源: DOAJ

【 摘 要 】

Milk production phenotypes are the main focus of genetic selection in dairy herds, and although there are many genes identified as related to the biology of these traits in pure breeds, little is known about crossbreed animals. This study aimed to identify potential genes associated with the 305-day milk yield in 337 crossbreed Gir × Holstein (Girolando) animals. Milk production records were genotyped for 45,613 single-nucleotide polymorphisms (SNPs). This dataset was used for a genome-wide association study (GWAS) using the 305-day milk yield adjusted for the fixed effects of herd and year and linear and quadratic effects of age at calving (in days) and calving factor averaged per animal. Genes within the significant SNPs were retrieved from the Bos taurus ARS-UCD1.2 assembly (bosTau9) for gene ontology analysis. In summary, the GWAS identified 52 SNPs associated [p ≤ 10–4, false discovery rate (FDR) = 8.77%] with milk production, including NUB1 and SLC24A2, which were previously described as related to milk production traits in cattle. The results suggest that SNPs associated mainly with NUB1 and SLC24A2 could be useful to understand milk production in Girolando and used as predictive markers for selecting genetic predisposition for milk yield in Girolando.

【 授权许可】

Unknown