Biostimulation of Microbial Communities from Malaysian Agricultural Soil for Detoxification of Metanil Yellow Dye; a Response Surface Methodological Approach

Sustainability

SitiSalwa Abd Gani¹  MohdYunus Abd Shukor²  UswatunHasanah Zaidan²  Khairil Mahmud³  FatinNatasha Amira Muliadi⁴  MohdIzuan Effendi Halmi⁴  SamsuriBin Abdul Wahid⁴ 

[1] Department of Agricultural Technology, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia;Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia;Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia;Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia;

关键词: bioremediation;    dye decolorization;    mixed culture;    metanil yellow;    response surface methodology;   

DOI  :  10.3390/su13010138

来源: DOAJ

【 摘 要 】

In the present study, a mixed culture from a local agricultural soil sample was isolated for Metanil Yellow (MY) dye decolorization. The metagenomic analysis confirmed that 42.6% has been dominated by genus Bacillus, while Acinetobacter (14.0%) is present in the microbial communities of the mixed culture. For fungi diversity analysis, around 97.0% was “unclassified” fungi and 3% was Candida. The preliminary investigation in minimal salt media (MSM) showed that 100% decolorization was achieved after 24 h of incubation. Response surface methodology (RSM) was successfully applied using Box-Behnken design (BBD) to study the effect of four independent parameters—MY dye concentration, glucose concentration, ammonium sulfate concentration, and pH—on MY dye decolorization by the mixed bacterial culture. The optimal conditions predicted by the desirability function were 73 mg/L of MY, 1.934% glucose, 0.433 g/L of ammonium sulfate, and a pH of 7.097, with 97.551% decolorization The correlation coefficients (R² and R² adj) of 0.913 and 0.825 indicate that the established model is suitable to predict the effectiveness of dye decolorization under the investigated condition. The MY decolorization of the mixed bacterial culture was not affected by the addition of heavy metals in the growth media. Among the 10 heavy metals tested, only copper gave 56.19% MY decolorization, whereas the others gave almost 100% decolorization. The decolorization potential of the mixed bacterial culture indicates that it could be effective for future bioremediation of soil-contaminated sites and treatment solutions of water bodies polluted with the MY dye.

【 授权许可】

Unknown