Circulating argonaute-bound microRNA-126 reports vascular dysfunction and treatment response in acute and chronic kidney disease

iScience

Matthew A. Bailey¹  James W. Dear¹  A. D. Bastiaan Vliegenthart¹  Alicja Czopek¹  Laura Rivoli¹  Robert W. Hunter¹  Tariq E. Farrah¹  Wilna Oosthuyzen¹  Neeraj Dhaun¹  David J. Webb¹  Kathleen M. Scullion¹ 

[1] University/British Heart Foundation Centre of Research Excellence, Centre for Cardiovascular Science, University of Edinburgh, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK;

关键词: Molecular Physiology;    Molecular Genetics;    Molecular Biology;    Immunology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Vascular and kidney dysfunction commonly co-exist. There is a need for biomarkers of vascular health. Circulating microRNAs are biomarkers; miR-126 is endothelial cell-enriched. We measured circulating miR-126 in rats with nephrotoxic nephritis (NTN) and humans with acute endothelial and renal injury (vasculitis associated with autoantibodies to neutrophil cytoplasm antigens (ANCAs)). We compared these findings to those from patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD) and explored the relationship between miR-126 and vascular dysfunction. In NTN, miR-126 was reduced. In ANCA vasculitis (N = 70), pre-treatment miR-126 was reduced compared to health (N = 60) (88-fold). miR-126 increased 3.4-fold post-treatment but remained lower than in health (∼26-fold). Argonaute 2-bound miR-126 increased with ANCA vasculitis treatment. miR-126 did not differ between CKD (N = 30) and health but its concentration correlated with endothelial dysfunction. miR-126 was reduced in ESRD (N = 15) (∼350 fold). miR-126 may be a marker of vascular inflammation and could aid decision-making.

【 授权许可】

Unknown