Wheat ATI CM3, CM16 and 0.28 Allergens Produced in Pichia Pastoris Display a Different Eliciting Potential in Food Allergy to Wheat ‡

Plants

Mickael Lafond¹  Thierry Giardina¹  Silvio Tundo²  Francesco Sestili²  Raviraj Kalunke²  Stefania Masci²  Martine Morisset³  Roberta Lupi⁴  Sandra Denery-Papini⁴  Colette Larré⁴ 

[1] Aix Marseille Univ, CNRS, Centrale Marseille, iSm2, Marseille, France;Department of Agriculture and Forest Science (DAFNE), University of Tuscia, Via S. C. de Lellis snc, 01100 Viterbo, Italy;Service d’Allergologie - CHU ANGERS, 4 rue Larrey 49933 Angers Cedex 9, France;UR 1268 BIA (Biopolymers, Interactions, Assemblies) INRA, 44300 Nantes, France;

关键词: wheat;    alpha-amylase/trypsin inhibitor;    heterologous expression;    Pichia pastoris;    allergy;   

DOI  :  10.3390/plants7040101

来源: DOAJ

【 摘 要 】

Although wheat is a staple food for most of the human population, some of its components trigger adverse reactions. Among wheat components, the alpha-amylase/trypsin inhibitors (ATI) are important triggers of several allergies and activators of innate immunity. ATI are a group of exogenous protease inhibitors and include several polypeptides. The three ATI polypeptides named CM3, CM16 and 0.28 are considered major allergens, and might also play a role in other common wheat-related pathologies, such as Non Celiac Wheat Sensitivity and even Celiac Disease. On this basis, we pointed to obtain high amounts of them in purity and to evaluate their allergenicity potential. We thus isolated the mRNA corresponding to the three ATI genes CM3, CM16 and 0.28 from 28 days post-anthesis wheat kernels and the corresponding cDNAs were used for heterologous expression in Pichia pastoris. The three purified proteins were tested in degranulation assay against human sera of patients with food allergy to wheat. A large range of degranulation values was observed for each protein according to the sera tested. All of the three purified proteins CM3, CM16 and 0.28 were active as allergens because they were able to induce basophils degranulation on wheat allergic patients’ sera, with the highest values of β-hexosaminidase release observed for CM3 protein.

【 授权许可】

Unknown