Rhizosphere Microbial Community Diversity and Function Analysis of Cut Chrysanthemum During Continuous Monocropping

Frontiers in Microbiology

Xu Jiang¹  Yiqing Zhou¹  Delong Kong¹  Zhiyong Ruan²  Rebecca E. Parales⁴  Xin Zhao⁵  Kexin Yang⁵  Zhiye Wang⁶  Tan Wang⁷  Lin Li⁷  Qingyun Ma⁷ 

[1] CAAS-CIAT Joint Laboratory in Advanced Technologies for Sustainable Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China;College of Life Sciences, Yantai University, Yantai, China;College of Resources and Environment, Tibet Agricultural and Animal Husbandry University, Linzhi, China;Department of Microbiology and Molecular Genetics, College of Biological Sciences, University of California, Davis, Davis, CA, United States;Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China;Key Laboratory of Microbial Resources Exploitation and Application of Gansu Province, Institute of Biology, Gansu Academy of Sciences, Lanzhou, China;State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China;

关键词: cut chrysanthemum;    rhizosphere;    community structure;    soil physical and chemical property decline;    continuous cropping barrier;   

DOI  :  10.3389/fmicb.2022.801546

来源: DOAJ

【 摘 要 】

As an ornamental flower crop, the long-term continuous monocropping of cut chrysanthemum causes frequent occurrence of diseases, seriously affecting the quality of cut chrysanthemum. The rhizosphere microbial community plays an important role in maintaining the healthy growth of plants, whereas the composition and dynamics of rhizosphere microbial community under continuous monocropping of cut chrysanthemum have not been fully revealed. In this study, the Illumina MiSeq high-throughput sequencing platform was used to monitor the dynamic changes of rhizosphere microbial communities in four varieties of cut chrysanthemum during 0–3 years of monocropping, and the soil physicochemical properties were also determined. Results showed that continuous monocropping significantly increased the fungal community richness and altered the profiles of the bacterial and fungal communities, leading to variation of community beta-diversity. With the increase of continuous cropping time, biocontrol bacteria decreased, while some plant pathogenic fungi were enriched in the rhizosphere of cut chrysanthemum. FAPROTAX-based functional prediction showed that the abundance of gene related to nitrogen and sulfur metabolism and chitin lysis was reduced in the rhizosphere of cut chrysanthemum. FUNGuild-based fungal function prediction showed that plant pathogenic fungal taxa were increasing in the rhizosphere of cut chrysanthemum, mainly Acremonium, Plectosphaerellaceae, Fusarium, and Cladosporium. Continuous cropping also reduced the content of ammonium nitrogen and increased soil salinity, resulting in deterioration of soil physical and chemical properties, which, together with the transformation of rhizosphere microbial community, became part of the reasons for the continuous cropping obstacle of cut chrysanthemum.

【 授权许可】

Unknown