Proteolytic Activity of Edible Spruce Morchella esculenta

Folia Veterinaria

Bedlovičová Z.¹  Strapáč I.¹  Baranová M.² 

[1] Department of Chemistry, Biochemistry and Biophysic, Institute of Pharmaceutical Chemistry;Department of Food Hygiene and Technology, Institute of Milk Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81Košice, Slovakia;

关键词: casein substrate;    morchella esculenta;    mushrooms;    non-specific proteolytic activity;   

DOI  :  10.2478/fv-2019-0020

来源: DOAJ

【 摘 要 】

This study focused on the determination of non-specific proteolytic activity of edible spruce Morchella esculenta in water extract, phosphate-buffered saline (PBS) solution (pH = 7.5) extract and a suspension prepared from 200 mg DW (dry weight) of edible spruce in PBS solution (pH 7.5). A clear casein solution was used as a substrate. The absorbances were measured in quartz cuvettes at the wavelength of 280 nm against a blank with zero concentration of trypsin. Non-specific proteolytic activity was expressed as trypsin equivalents per kilogram of mushroom dry weight (mg.kg−1 DW). All of the extracts demonstrated non-specific enzymatic activity. The highest activity was observed in the PBS suspension and the lowest enzymatic activity was measured in the water extract of the Morchella esculenta fungi. The non-specific proteolytic activity decreased in the following order: PBS suspension extract (pH 7.5; 22.9 mg.kg−1 DW), followed by PBS extract (pH 7.5; 13.6 mg.kg−1 DW) and finally the water extract (10.94 mg.kg−1 DW).

【 授权许可】

Unknown