Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates

Journal of Fungi

Guillermo Quindós¹  Iker De-la-Pinta¹  Uwe Groß²  Oliver Bader²  Emilia Gómez-Molero²  Michael Weig²  PietW. J. de Groot³  Jordan Fernández-Pereira³ 

[1] Department of Immunology, Microbiology and Parasitology, School of Medicine and Nursing, Universidad del País Vasco (UPV/EHU), 48940 Bilbao, Spain;Institute for Medical Microbiology, University Medical Center Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany;Regional Center for Biomedical Research, Castilla-La Mancha Science & Technology Park, University of Castilla–La Mancha, 02008 Albacete, Spain;

关键词: Candida parapsilosis;    biofilm;    colony morphology;    drug susceptibility;   

DOI  :  10.3390/jof7010033

来源: DOAJ

【 摘 要 】

Candida parapsilosis is a frequent cause of fungal bloodstream infections, especially in critically ill neonates or immunocompromised patients. Due to the formation of biofilms, the use of indwelling catheters and other medical devices increases the risk of infection and complicates treatment, as cells embedded in biofilms display reduced drug susceptibility. Therefore, biofilm formation may be a significant clinical parameter, guiding downstream therapeutic choices. Here, we phenotypically characterized 120 selected isolates out of a prospective collection of 215 clinical C. parapsilosis isolates, determining biofilm formation, major emerging colony morphotype, and antifungal drug susceptibility of the isolates and their biofilms. In our isolate set, increased biofilm formation capacity was independent of body site of isolation and not predictable using standard or modified European Committee on Antimicrobial Susceptibility Testing (EUCAST) drug susceptibility testing protocols. In contrast, biofilm formation was strongly correlated with the appearance of non-smooth colony morphotypes and invasiveness into agar plates. Our data suggest that the observation of non-smooth colony morphotypes in cultures of C. parapsilosis may help as an indicator to consider the initiation of anti-biofilm-active therapy, such as the switch from azole- to echinocandin- or polyene-based strategies, especially in case of infections by potent biofilm-forming strains.

【 授权许可】

Unknown