期刊论文详细信息
International Journal of Fertility and Sterility
The Effect of Imbalanced Progesterone Receptor-A/-B Ratio on Gelatinase Expressions in Endometriosis
Parvaneh Afsharian1  Maryam Shahhoseini2  Reza Aflatoonian3  Azadeh Ghaheri3  Sepideh Mousazadeh4 
[1] 4Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for ReproductiveBiomedicine, ACECR, Tehran, Iran;Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproduc- tive Biomedicine, ACECR, Tehran, Iran;Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine,ACECR, Tehran, Iran;Department of Genetics, School of Natural Sciences, University of Tabriz, Tabriz, Iran;
关键词: endometriosis;    gelatinases;    progesterone;    progesterone receptor;   
DOI  :  10.22074/ijfs.2019.5604
来源: DOAJ
【 摘 要 】

Background Gelatinases degrade extracellular matrix (ECM) components to allow for physiological remodeling and contribute to pathological tissue destruction in endometriosis. It is known that the function of gelatinases is resistant to suppression by progesterone in endometriosis. The ability of progesterone to impact gene expression depends on the progesterone receptor-A/-B (PR-A/PR-B) ratio. An imbalanced PR-A/PR-B ratio in endometriotic tissue may be the result of the differential expression of MMP-2 and MMP-9, which could be important in the etiology and pathogenesis of the disease. Hence, we decided to study the association of PR-A/PR-B ratio and gelatinases expression in endometriosis. Materials and Methods In this prospective case-control study, we enrolled 40 women, 20 in the case group who were diagnosed with stage III/IV endometriosis and 20 normal subjects without endometriosis (controls) who referred to Royan Institute, Tehran, Iran during 2013-2014. We obtained 60 tissue samples [ectopic (n=20), eutopic (n=20), and normal endometrium (n=20)]. RNA was extracted from the tissue samples in order to analyze PR-A, PR-B, MMP-2, and MMP-9 mRNA levels through real-time polymerase chain reaction (PCR). Results There was significantly lower expression of the PR-B isoform in ectopic tissues compared to the control (P=0.002) and eutopic endometrium (P=0.006) tissues. PR-A expression was higher, but not significantly so, in the same ectopic and eutopic endometrium tissues compared to the control tissues (P=0.643). There was significant over- expression of MMP-9 in ectopic samples compared to control (P=0.014) and eutopic endometrium (P=0.012) samples. The PR-A/PR-B ratio was not significantly higher in either eutopic or ectopic samples compared to the control samples (P=0.305). Conclusion Our findings support an altered PR-B expression in endometriosis, which may be associated with MMP-9 overexpression. This finding can be important for disease pathogenesis.

【 授权许可】

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