Establishment of Self-incompatibility Gene cDNA Microarray to Identify S-genotypes of Pyrus pyrifolia

Horticultural Plant Journal

Hu Hongju¹  Zhang Jingguo¹  Tan Xiaofeng²  Zhang Lin²  Jiang Nan³ 

[1]Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences, Wuhan 430064, China

[2]Key Laboratory of Cultivation and Protection for Non-wood Forest of Ministry of Education, the Key Laboratory of Non-wood Forest Product of Forestry Ministry, Central-south University of Forestry and Technology, Changsha 410004, China

[3]School of Packing and Material Engineering, Hunan University of Technology, Zhuzhou, Hunan 412007, China

关键词: pear;    self-incompatibility;    S-genotype;    cDNA microarray;    oligonucleotide gene chip;   

DOI  :  10.16420/j.issn.2095-9885.2016-0019

来源: DOAJ

【 摘 要 】

Based on the cDNA sequences from hyper variable (HV) regions of identified 52 S-alleles in Oriental pear cultivars, S-RNase cDNA probes were designed, and a cDNA microarray for S-RNase detections was established. Each microarray contained 240 sites from 55 cDNA probes, including all specific cDNA sequences from the HV regions of the S-alleles. Using the cDNA of pistils of tested pear cultivars as template and Cy3 fluorescently labeling primers by PCR amplification, microarray hybridization detected the S-genotype of each pear cultivar. The genotypes inferred from the cDNA microarray hybridization signals of pear cultivars such as ‘Lijiang Huangsuanli’, ‘Xiuyu’, ‘Midu Yuli’, ‘Baimianli’, and ‘Deshengxiang’ were similar to the known genotypes of all tested cultivars. The S-RNase cDNA microarrays and the oligonucleotide gene chips were then used to conduct parallel testing of 24 P. pyrifolia cultivars with unknown S-genotypes. In conclusion, the construction of cDNA microarrays has further improved the pear S-RNase detection platform.

【 授权许可】

Unknown