DNA Methylation-Based Panel Predicts Survival of Patients With Clear Cell Renal Cell Carcinoma and Its Correlations With Genomic Metrics and Tumor Immune Cell Infiltration

Frontiers in Cell and Developmental Biology

Xinghuan Wang²  Xiao-Ping Liu²  Tongzu Liu²  Lingao Ju³  Sheng Li³  Chen Chen³ 

[1] Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, China;Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China;Human Genetics Resource Preservation Center of Wuhan University, Wuhan, China;

关键词: clear cell renal cell carcinoma;    methylation CpG island;    prognostication panel;    elastic net;    survival analysis;   

DOI  :  10.3389/fcell.2020.572628

来源: DOAJ

【 摘 要 】

DNA methylation based prognostic factor for patients with clear cell renal cell carcinoma (ccRCC) remains unclear. In the present study, we identified survival-related DNA methylation sites based on the differentially methylated DNA CpG sites between normal renal tissue and ccRCC. Then, these survival-related DNA methylation sites were included into an elastic net regularized Cox proportional hazards regression (CoxPH) model to build a DNA methylation-based panel, which could stratify patients into different survival groups with excellent accuracies in the training set and test set. External validation suggested that the DNA methylation-based panel could effectively distinguish normal controls from tumor samples and classify patients into metastasis group and non-metastasis group. The nomogram containing DNA methylation-based panel was reliable in clinical settings. Higher total mutation number, SCNA level, and MATH score were associated with higher methylation risk. The innate immune, ratio between CD8+T cell versus Treg cell as well as Th17 cell versus Th2 cell were significantly decreased in high methylation risk group. In inclusion, we developed a DNA methylation-based panel which might be independent prognostic factor in ccRCC. Patients with higher methylation risk were associated genomic alteration and poor immune microenvironment.

【 授权许可】

Unknown