A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles

Antioxidants

Soo-Kyung Jun¹  Sang-Cheol Lee²  Hae-Won Kim²  Hae-Hyoung Lee²  Jung-Hwan Lee²  Jonathan Campbell Knowles²  Na-Hyun Lee²  Kapil D. Patel²  Jeong-Hui Park² 

[1] Department of Dental Hygiene, Hanseo University, Seosan 31962, Korea;Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan 31116, Chungcheongnam-do, Korea;

关键词: C2C12;    skeletal muscle differentiation;    selenium nanoparticle;    ROS;    antioxidant;   

DOI  :  10.3390/antiox10111727

来源: DOAJ

【 摘 要 】

Reactive oxygen species (ROS) are continuously produced by skeletal muscle during contractile activity and even at rest. However, the ROS generated from excessive exercise or traumatic damage may produce more ROS than can be neutralized by an antioxidant capacity, which can be harmful to muscle function. In particular, selenium is a known antioxidant that regulates physiological functions such as cell differentiation and anti-inflammatory function. In this study, we developed nano-sized antioxidative biomaterials using selenium to investigate the protective and differentiation effects against C2C12 myoblasts in an H₂O₂-induced oxidative stress environment. The selenium nanoparticles (SeNPs) were produced with a size of 35.6 ± 4.3 nm and showed antioxidant effects according to the 3,3′,5,5′-tetramethylbenzidine assay. Then, SeNPs were treated to C2C12 cells with or without H₂O₂. Our results showed that SeNPs reduced C2C12 apoptosis and intracellular ROS levels. Additionally, SeNPs effectively up-regulated in the presence of H₂O₂, MyoD, MyoG, α-actinin, and myosin heavy chain, which are well known to increase during myoblast differentiation as assayed by qRT-PCR, immunocytochemistry-staining, western blotting. These results demonstrate that SeNPs can accelerate differentiation with its protective effects from the ROS environment and can be applied to the treatment of skeletal muscle in a cellular redox environment.

【 授权许可】

Unknown