EFFECT OF TILETAMINE-ZOLAZEPAM AND MORPHINE IN RATS: LATENCY PERIOD, BIOCHEMISTRY AND HEMATOLOGICAL EVALUATION

Atas de Saúde Ambiental

Deise Carla Almeida Leite-Dellova¹  Márcia Cristina Menão²  Kelly Priscila Pandolfi³  Paulo César de Carvalho Ferreira⁴ 

[1]Departamento de Medicina Veterinária. Faculdade de Zootecnia e Engenharia de Alimentos. Av. Duque de Caxias Norte, 225, Jardim Elite, CEP 13635-900, Pirassununga-SP.

[2]Faculdades Metropolitanas Unidas – Complexo Educacional FMU. R. Ministro Nelson Hungria, 541, Real Parque, CEP 05690-050, São Paulo-SP.

[3]Médica veterinária autônoma

[4]Universidade de Santo Amaro (UNISA). R. José Portolano, 57, Jardim das Imbuias, CEP 04829-320, São Paulo-SP.

关键词: analgesia;    anestesia;    animais de laboratório;    hemograma;    perfil bioquímico.;   

DOI  :  

来源: DOAJ

【 摘 要 】

Researchers seek alternatives to minimize the pain and stress of laboratory animals. Procedures that promote pain should be performed under anesthesia and analgesia. The combination tiletamine-zolazepam (TZ) is used for rodent anesthesia, with advantages: easy administration and rapid induction and disadvantages: long recovery and variable analgesia. In this situation, morphine could provide an additional analgesia; however, the effects of TZ plus morphine have not been described in rats. The objective of this study was to determine the latency period of TZ plus morphine in rats Wistar male, and evaluate the biochemical and hematological parameters of this species, after anesthesia. TZ plus morphine were administered intramuscularly. Blood was obtained by cardiac puncture and laboratory tests were performed using standardized techniques. The results indicated that the anesthetic protocol was effective to anesthesia and analgesia in rats, due to the short latency period (6.26 ± 1.23 min, N = 50) and absence of responses to painful stimuli. Except for the higher urea, TZ plus morphine, practically, did not interfere in the biochemical parameters; but important hematological alterations were observed: low values for red blood cell count and hemoglobin concentration.

【 授权许可】

Unknown