Quinolone Signals Related to Pseudomonas Quinolone Signal-Quorum Sensing Inhibits the Predatory Activity of Bdellovibrio bacteriovorus

Frontiers in Microbiology

Ryutaro Hirano¹  Tae Moriya¹  Toshinari Maeda¹  Yuki Hoshiko¹  Yoshito Nishiyama¹  Rodolfo García-Contreras²  Kiwao Kadokami³  Luis Esaú López-Jácome⁴ 

[1] Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu, Japan;Department of Microbiology and Parasitology, Faculty of Medicine, UNAM, Mexico City, Mexico;Institute of Environmental Science and Technology, The University of Kitakyushu, Kitakyushu, Japan;Laboratory of Infectology, National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra, Mexico City, Mexico;

关键词: inhibition factors;    Escherichia coli;    quorum sensing;    Pseudomonas quinolone signal;    Pseudomonas aeruginosa;    Bdellovibrio bacteriovorus;   

DOI  :  10.3389/fmicb.2021.722579

来源: DOAJ

【 摘 要 】

Bdellovibrio bacteriovorus is one of the predatory bacteria; therefore, it can act as a novel “living antibiotic,” unlike the current antibiotics. Here the predation of Escherichia coli by B. bacteriovorus was inhibited in the presence of Pseudomonas aeruginosa. This study investigated whether P. aeruginosa-induced predation inhibition is associated with bacterial quorum sensing (QS). Each las, rhl, or pqs QS mutant in P. aeruginosa was used to check the predatory activity of E. coli cells using B. bacteriovorus. As a result, the predatory activity of B. bacteriovorus increased in a mutant pqs QS system, whereas wild-type PA14 inhibited the predatory activity. Moreover, the addition of 4-hydroxy-2-heptylquinoline (HHQ) or the analog triggered the low predatory activity of B. bacteriovorus and killed B. bacteriovorus cells. Therefore, a defensive action of P. aeruginosa against B. bacteriovorus is activated by the pqs QS system, which produces some quinolone compounds such as HHQ.

【 授权许可】

Unknown