Comparing passive leg raising and suprasystolic ankle occlusion responses to quantify age-related microcirculatory status

Journal Biomedical and Biopharmaceutical Research (BBR)

Hugo Ferreira¹  Henrique Silva²  L Monteiro Rodrigues³  Julia Bujan⁴ 

[1] Pharmacol. Sc Depart – Universidade de Lisboa, School of Pharmacy;CBIOS – Universidade Lusófona’s Research Center for Biosciences and Health Technologies;Faculty of Medicine, Universidad Alcalá de Henares;IBEB - Universidade de Lisboa, Faculty of Sciences;

关键词: Vascular ageing;    PLR;    suprasystolic occlusion;    LDF;    tcpO2;   

DOI  :  10.19277/bbr.12.2.118

来源: DOAJ

【 摘 要 】

The skin provides an easy accessible vascular network to assess microcirculation by laser Doppler flowmetry (LDF), and transcutaneous (tc) gasometry, widely known techniques used for this purpose. the objective of this study was to assess and compare the microcirculation status of different age subjects, knowing that the ageing process progressively affects macro and microcirculatory vessels. We’ve chosen to compare dynamical responses to two provocation tests – passive leg raising (PLR) and ankle occlusion - in 59 healthy subjects, 35 young (22.1 ± 3.7 years old.) in Group 1, and 24 older (50.8 ± 7.6 years old.) in Group 2, selected after informed consent. Local blood flow, tcpO2 and transepidermal water loss (TEWL) were measured in distal locations of the lower limb. PLR and ankle occlusion significantly reduced blood flow and tcpO2 in both groups, while no changes were found for TEWL. While the magnitude of the hyperemic response was found to be significantly reduced in group 2, no differences were found during ankle occlusion. tcpO2-dependent parameters were also significantly different between groups. These results seem to confirm the usefulness of these experimental models to distinguish the microcirculatory function of subjects with different ages, with PLR being more sensible in detecting age-related changes.

【 授权许可】

Unknown