Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical Cancer

International Journal of Molecular Sciences

Doru Diculescu¹  Carmen Bucuri¹  Andrei Mihai Malutan¹  Radu Mocan-Hognogi¹  Marina Dudea-Simon¹  Razvan Ciortea¹  Dan Mihu¹  Roxana Maria Cojocneanu²  Cristina Alexandra Ciocan²  Ioana Berindan-Neagoe²  Laura Ancuta Pop²  Cornelia Braicu²  Vasile Simon³ 

[1] 2nd Obstetrics and Gynecology Department, “Dominic Stanca” Obstetrics and Gynecology Clinic, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania;Research Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 15 Victor Babeș Street, 400012 Cluj-Napoca, Romania;Urology Department, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania;

关键词: cervical cancer;    cervical intraepithelial neoplasia;    long noncoding RNAs;    microRNA;   

DOI  :  10.3390/ijms23116054

来源: DOAJ

【 摘 要 】

Background: Cervical cancer is one of the most common malignancies in women in terms of prevalence and mortality. Cervical cancer has some particularities that distinguish it from any other oncologic pathology: first, it is completely preventable by prompt detection of its precursor, cervical intraepithelial neoplasia (CIN); second, the Human Papillomavirus (HPV) infection is a known etiological agent; third, the mean age at diagnosis is much lower than in other oncologic conditions, as a consequence of the sexually-transmitted HPV. Methods: We evaluated the expression level of several long noncoding RNAs and a microRNA in samples from 30 patients with CIN, 9 with cervical cancer and 38 normal samples using qRT-PCR technology. Results: We observed higher expression levels for MEG3, DAPK1, MLH1 and MALAT1 in CIN samples than in normal samples, whereas TIMP3 and SOX1 had lower expression levels. For cancer samples, DAPK1, MLH1 and MALAT1 had higher expression, and MEG3, TIMP3 and SOX1 had lower expression when compared to normal samples. In the case of CIN versus cancer samples, only MEG3 gene showed a statistically significant difference. The expression of miR-205-5p was lower in both CIN and cancer samples compared to normal samples. Conclusion: Decreased MEG3 expression could be considered an alarm signal in the transition from a premalignant cervical lesion to invasive cancer, while altered expression levels of TIMP3, SOX1, MLH1, MALAT1 and miR-205-5p could serve as early biomarkers in the diagnosis of premalignant cervical lesions. Future studies, including a larger number of patients with CIN, will be of particular importance in validating these observations.

【 授权许可】

Unknown