Synergistic Effect of Abietic Acid with Oxacillin against Methicillin-Resistant Staphylococcus pseudintermedius

Antibiotics

Marina DellaGreca¹  Adriana Vollaro²  MariaR. Catania²  Elisabetta Buommino³  Francesca Lembo³  Luisa De Martino⁴  FrancescaP. Nocera⁴ 

[1] Department of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy;Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II,80131 Naples, Italy;Department of Pharmacy, University of Naples Federico II, 80131 Naples, Italy;Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy;

关键词: abietic acid;    Staphylococcus pseudintermedius;    methicillin-resistant Staphylococcus pseudintermedius;    methicillin-susceptible Staphylococcus pseudintermedius;    antimicrobial activity;    synergistic interaction;   

DOI  :  10.3390/antibiotics10010080

来源: DOAJ

【 摘 要 】

Resin acids are valued in traditional medicine for their antiseptic properties. Among these, abietic acid has been reported to be active against methicillin-resistant Staphylococcus aureus (MRSA) strains. In veterinary healthcare, the methicillin-resistant Staphylococcus pseudintermedius (MRSP) strain is an important reservoir of antibiotic resistance genes including mecA. The incidence of MRSP has been increasing, and treatment options in veterinary medicine are partial. Here, we investigated the antimicrobial and antibiofilm properties of abietic acid against three MRSP and two methicillin-susceptible Staphylococcus pseudintermedius (MSSP) strains, isolated from diseased pet animals and human wound samples. Abietic acid showed a significant minimal inhibitory concentration (MIC) value ranging from 32 to 64 μg/mL (MRSPs) and 8 μg/mL (MSSP). By checkerboard method we demonstrated that abietic acid increased oxacillin susceptibility of MRSP strains, thus showing a synergistic interaction with oxacillin. Abietic acid was also able to contrast the vitality of treated MSSP and MRSP1 biofilms at 20 μg/mL and 40 μg/mL, respectively. Finally, the compound moderately reduced mecA, mecR1 and mec1 gene expression. In conclusion, the results here reported demonstrate the antimicrobial activity of abietic acid against MRSP and support the use of this compound as a potential therapeutic agent to be used in combinatorial antibiotic therapy.

【 授权许可】

Unknown