Profiling of Seed Proteome in Pea (Pisum sativum L.) Lines Characterized with High and Low Responsivity to Combined Inoculation with Nodule Bacteria and Arbuscular Mycorrhizal Fungi

Molecules

Andrea Sinz¹  Alena Soboleva²  VladimirA. Zhukov²  Tatiana Mamontova²  Elena Lukasheva²  IgorA. Tikhonovich³  OksanaY. Shtark³  GulnaraA. Akhtemova³  AlexeyM. Afonin³  AntonS. Sulima³  Christian Ihling⁴  Andrej Frolov⁴  MariaN. Povydysh⁵ 

[1] D Department, Saint-Petersburg State Chemical and Pharmaceutical University, 197376 St. Petersburg, Russia;Department of Biochemistry, St. Petersburg State University, 199178 St. Petersburg, Russia;Department of Biotechnology, All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia;Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther Universität Halle-Wittenberg, 06120 Halle (Saale), Germany;;R&

关键词: arbuscular mycorrhiza;    beneficial soil microorganisms;    combined inoculation;    inoculation efficiency;    LC-MS;    pea (Pisum sativum L.);    proteomics;    rhizobial symbiosis;    seed proteome;   

DOI  :  10.3390/molecules24081603

来源: DOAJ

【 摘 要 】

Legume crops represent the major source of food protein and contribute to human nutrition and animal feeding. An essential improvement of their productivity can be achieved by symbiosis with beneficial soil microorganisms—rhizobia (Rh) and arbuscular mycorrhizal (AM) fungi. The efficiency of these interactions depends on plant genotype. Recently, we have shown that, after simultaneous inoculation with Rh and AM, the productivity gain of pea (Pisum sativum L) line K-8274, characterized by high efficiency of interaction with soil microorganisms (EIBSM), was higher in comparison to a low-EIBSM line K-3358. However, the molecular mechanisms behind this effect are still uncharacterized. Therefore, here, we address the alterations in pea seed proteome, underlying the symbiosis-related productivity gain, and identify 111 differentially expressed proteins in the two lines. The high-EIBSM line K-8274 responded to inoculation by prolongation of seed maturation, manifested by up-regulation of proteins involved in cellular respiration, protein biosynthesis, and down-regulation of late-embryogenesis abundant (LEA) proteins. In contrast, the low-EIBSM line K-3358 demonstrated lower levels of the proteins, related to cell metabolism. Thus, we propose that the EIBSM trait is linked to prolongation of seed filling that needs to be taken into account in pulse crop breeding programs. The raw data have been deposited to the ProteomeXchange with identifier PXD013479.

【 授权许可】

Unknown