Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

International Journal of Molecular Sciences

Ewa Kijeńska-Gawrońska¹  PawełK. Włodarski²  Wiktor Paskal²  Tomasz Dębski³  Katarzyna Siennicka³  Aleksandra Zołocińska³  Anna Słysz³  Zygmunt Pojda³  Wojciech Święszkowski⁴ 

[1] Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, Poleczki 19, 02-822 Warsaw, Poland;Centre for Preclinical Research, Department of Methodology, Medical University of Warsaw, Banacha 1b, 02-097 Warsaw, Poland;Department of Regenerative Medicine, Maria Sklodowska-Curie National Research Institute of Oncology, Wawelska 15B, 02-034 Warsaw, Poland;Materials Design Division, Faculty of Materials Science and Engineering, Warsaw University of Technology, Woloska 141, 02-507 Warsaw, Poland;

关键词: nerve reconstruction;    conduit;    ASCs;    P(LLA-CL)-COL-PANI;    peripheral nerve injury;    nerve gap;   

DOI  :  10.3390/ijms22115588

来源: DOAJ

【 摘 要 】

The aim was to examine the efficiency of a scaffold made of poly (L-lactic acid)-co-poly(ϵ-caprolactone), collagen (COL), polyaniline (PANI), and enriched with adipose-derived stem cells (ASCs) as a nerve conduit in a rat model. P(LLA-CL)-COL-PANI scaffold was optimized and electrospun into a tubular-shaped structure. Adipose tissue from 10 Lewis rats was harvested for ASCs culture. A total of 28 inbred male Lewis rats underwent sciatic nerve transection and excision of a 10 mm nerve trunk fragment. In Group A, the nerve gap remained untouched; in Group B, an excised trunk was used as an autograft; in Group C, nerve stumps were secured with P(LLA-CL)-COL-PANI conduit; in Group D, P(LLA-CL)-COL-PANI conduit was enriched with ASCs. After 6 months of observation, rats were sacrificed. Gastrocnemius muscles and sciatic nerves were harvested for weight, histology analysis, and nerve fiber count analyses. Group A showed advanced atrophy of the muscle, and each intervention (B, C, D) prevented muscle mass decrease (p < 0.0001); however, ASCs addition decreased efficiency vs. autograft (p < 0.05). Nerve fiber count revealed a superior effect in the nerve fiber density observed in the groups with the use of conduit (D vs. B p < 0.0001, C vs. B p < 0.001). P(LLA-CL)-COL-PANI conduits with ASCs showed promising results in managing nerve gap by decreasing muscle atrophy.

【 授权许可】

Unknown