A fungal member of the Arabidopsis thaliana phyllosphere antagonizes Albugo laibachii via a GH25 lysozyme

eLife

Priyamedha Sengupta¹  Samuel Kroll²  Katharina Eitzen²  Gunther Doehlemann³  Eric Kemen³ 

[1] Max Planck Institute for Plant Breeding Research, Cologne, Germany;Institute for Plant Sciences and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Center for Molecular Biosciences, Cologne, Germany;Max Planck Institute for Plant Breeding Research, Cologne, Germany;

关键词: microbial antagonism;    albugo laibachii;    ustilaginales;    arabidopsis;    transcriptomics;    effectors;   

DOI  :  10.7554/eLife.65306

来源: DOAJ

【 摘 要 】

Plants are not only challenged by pathogenic organisms but also colonized by commensal microbes. The network of interactions these microbes establish with their host and among each other is suggested to contribute to the immune responses of plants against pathogens. In wild Arabidopsis thaliana populations, the oomycete pathogen Albugo laibachii plays an influential role in structuring the leaf phyllosphere. We show that the epiphytic yeast Moesziomyces bullatus ex Albugo on Arabidopsis, a close relative of pathogenic smut fungi, is an antagonistic member of the A. thaliana phyllosphere, which reduces infection of A. thaliana by A. laibachii. Combination of transcriptomics, reverse genetics, and protein characterization identified a GH25 hydrolase with lysozyme activity as a major effector of this microbial antagonism. Our findings broaden the understanding of microbial interactions within the phyllosphere, provide insights into the evolution of epiphytic basidiomycete yeasts, and pave the way for novel biocontrol strategies.

【 授权许可】

Unknown