Buffering the pH of the culture medium does not extend yeast replicative lifespan [v1; ref status: indexed, http://f1000r.es/20w]

F1000Research

Joey Feng¹  Mekedes Lemma¹  Torin Stetina¹  Janet Lee¹  Ben Zipkin¹  Jillian R Neault¹  Nathan Schurman¹  Jacob Mouser¹  Daniel T Carr¹  Anna Shemorry¹  Matt Kaeberlein¹  Ha Doan¹  Herman Tung¹  Edward Oudanonh¹  Tina Nguyen¹  Brian M Wasko¹ 

[1] Department of Pathology, University of Washington, Seattle, WA, 98195, USA;

关键词: Microbial Growth & Development;    Microbial Physiology & Metabolism;   

DOI  :  10.12688/f1000research.2-216.v1

来源: DOAJ

【 摘 要 】

During chronological aging of budding yeast cells, the culture medium can become acidified, and this acidification limits cell survival.  As a consequence, buffering the culture medium to pH 6 significantly extends chronological life span under standard conditions in synthetic medium.  In this study, we assessed whether a similar process occurs during replicative aging of yeast cells.  We find no evidence that buffering the pH of the culture medium to pH levels either higher or lower than the initial pH of the medium is able to significantly extend replicative lifespan.  Thus, we conclude that, unlike chronological life span, replicative life span is not limited by acidification of the culture medium or by changes in the pH of the environment.

【 授权许可】

Unknown