Solution Structure of the Circular γ-Domain Analog from the Wheat Metallothionein Ec-1

Molecules

Eva Freisinger¹  Silke Johannsen¹  Katsiaryna Tarasava¹ 

[1] Institute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, Zurich CH-8057, Switzerland;

关键词: plant metallothioneins;    metal-thiolate cluster;    backbone cyclization;    flexibility reduction;    NMR spectroscopy;   

DOI  :  10.3390/molecules181114414

来源: DOAJ

【 摘 要 】

The first cyclic analog of a metallothionein (MT) was prepared and analyzed by UV and (magnetic) circular dichroism spectroscopy, ESI-MS as well as NMR spectroscopy. Results reveal that the evaluated cyclic g-Ec-1 domain of the wheat MT Ec-1 retains its ability to coordinate two Zn(II) or Cd(II) ions and adopts a three-dimensional structure that is highly similar to the one of the linear wild-type form. However, the reduced flexibility of the protein backbone facilitates structure solution significantly and results in a certain stabilization of metal binding to the protein.

【 授权许可】

Unknown