Quantification of viable spray-dried potential probiotic lactobacilli using real-time PCR

Archives of Biological Sciences

关键词: Spray-drying;    potential probiotic;    real-time PCR;    propidium monoazide;   

DOI  :  10.2298/ABS1204465R

来源: DOAJ

【 摘 要 】

The basic requirement for probiotic bacteria to be able to perform expectedpositive effects is to be alive. Therefore, appropriate quantificationmethods are crucial. Bacterial quantification based on nucleic acid detectionis increasingly used. Spray-drying (SD) is one of the possibilities toimprove the survival of probiotic bacteria against negative environmentaleffects. The aim of this study was to investigate the survival of spray-driedLactobacillus plantarum 564 and Lactobacillus paracasei Z-8, and toinvestigate the impact on some probiotic properties caused by SD of bothtested strains. Besides the plate count technique, the aim was to examine thepossibility of using propidium monoazide (PMA) in combination with real-timepolymerase chain reaction (PCR) for determining spray-dried tested strains.The number of intact cells, Lb. plantarum 564 and Lb. paracasei Z-8, wasdetermined by real-time PCR with PMA, and it was similar to the number ofinvestigated strains obtained by the plate count method. Spray-dried Lb.plantarum 564 and Lb. paracasei Z-8 demonstrated very good probiotic ability.It may be concluded that the PMA real-time PCR determination of the viabilityof probiotic bacteria could complement the plate count method and SD may be acost-effective way to produce large quantities of some probiotic cultures.[Projekat Ministarstva nauke Republike Srbije, br. 046010]

【 授权许可】

Unknown