FKBP25 Regulates Meiotic Apparatus During Mouse Oocyte Maturation

Frontiers in Cell and Developmental Biology

Qiang Wang¹  Danni Wang²  Yongan Xin²  Hongzheng Sun²  Longsen Han²  Shoubin Tang²  Juan Ge²  Jiaqi Zhang²  Zhenyue Huang²  Congyang Li² 

[1] Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China;State Key Laboratory of Reproductive Medicine, Suzhou Municipal Hospital, Nanjing Medical University, Nanjing, China;

关键词: oocyte;    meiosis;    FKBP25;    maternal aging;    reproduction;   

DOI  :  10.3389/fcell.2021.625805

来源: DOAJ

【 摘 要 】

FK506 binding proteins 25 (FKBP25) has been shown to function in ribosome biogenesis, chromatin organization, and microtubule stability in mitosis. However, the role of FKBP25 in oocyte maturation has not been investigated. Here, we report that oocytes with FKBP25 depletion display abnormal spindle assembly and chromosomes alignment, with defective kinetochore-microtubule attachment. Consistent with this finding, aneuploidy incidence is also elevated in oocytes depleted of FKBP25. Importantly, FKBP25 protein level in old oocytes is significantly reduced, and ectopic expression of FKBP25 could partly rescue the aging-associated meiotic defects. In addition, by employing site-specific mutagenesis, we identify that serine 163 is a major, if not unique, phosphorylation site modulating the action of FKBP25 on meiotic maturation. In summary, our data indicate that FKBP25 is a pivotal factor for determining oocyte quality, and may mediate the effects of maternal aging on female reproduction.

【 授权许可】

Unknown