In vitro effect of different cytokinin types (BAP, TDZ) on two different Ocimum basilicum cultivars explants

International Journal of Horticultural Science

M. G.Fári¹  E. Kurucz²  E. Enkhbileg² 

[1] University of Debrecen, Faculty of Agricultural and Food Sciences and Environmental Management, Department of Botany, Biotechnology and Plant Physiology;University of Debrecen, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Plant Sciences, 4032 Debrecen, Böszörményi út 138, Hungary;

关键词: sweet basil;    Ocimum basilicum L.;    In vitro;    cytokinin effect;    BAP (6-Benzylaminopurine);    TDZ (Thidiazuron);   

DOI  :  10.31421/IJHS/25/3-4/3930

来源: DOAJ

【 摘 要 】

Ocimum basilicum L. (sweet basil) is an economically and ethnobotanically important aromatic, medicinal, ornamental and culinary herb, with a very wide gene pool, that is sensitive to cold and prone to several plant pathogens that can demolish harvest and lessen yield. In this research, the effects of BAP (6-Benzylaminopurine) and TDZ (Thidiazuron) on different genotypes for in vitro cloning were determined, in order to provide a detailed protocol guide concerning Ocimum basilicum L. propagation. The results from the O. basilicum seed propagations revealed that the best condition for the secondary shoot growth is with 5.0 mg/l TDZ or 1.5 mg/l BAP on all types of explants except the root, the secondary root growth can be obtained on all types explant with any BAP concentration and all cytokinins can induce callus on all types of explants. On the whole, it shows that multiple secondary shoot induction and regeneration in Ocimum basilicum L. is regulated by appropriate cytokinin concentration.

【 授权许可】

Unknown